Tissue Microarray (TMA): a revolution in Tissue - based research
ABSTRACT
Tissue microarray (TMA) is a recent innovation in the field of pathology, which is now available in Tissue Diagnostic and Cancer Research Institute, Emek Medical Center. Nowadays this automated technique became a favorite tool in tissue diagnostic researches. Most studies on tissues, deal with proteins identification using immunohistochemistry and immunofluorescence, which utilize the main part of the research budget. TMA module reduces the immunostain's cost in hundreds percent, by including many tissues samples on one slide. The TMA system (3DHISTECH) includes TMA Grand Master, Scanner and analytical softwares which allow a significant reduction in cost and time together with a major improvement of accuracy and quality.
What is TMA?
A tissue microarray contains many small representative tissue samples from hundreds of different cases assembled on a single histologic block/slide, and therefore allows high throughput analysis of multiple specimens at the same time. Tissue microarrays are paraffin blocks produced by extracting cylindrical tissue cores from different paraffin donor blocks and re-embedding these into a single recipient (microarray) block at defined array coordinates. Using this technique, hundreds of tissue samples can be presented onto a single slide for all kind of stains.
What are the parts of the TMA system?
TMA Grand Master
TMA Grand Master has world’s highest capacity of 72 blocks - 60 donor blocks at the same time and 12 recipient blocks. 4 different core diameters can be chosen by the researcher: 0.6 mm, 1 mm, 1.5 mm and 2 mm. The core sampling takes max. 12 seconds per core due to simultaneous loading, imaging, drilling and punching.
TMA Scanner- Pannoramic MIDI
The Pannoramic MIDI has a capacity of 12 slides which can be scanned in brightfield or up to 9-channel fluorescent scanning using monochrome camera for high quality. The loading, previewing barcode reading and scanning is automated.
TMA CASECENTER
The TMA casecenter is a server which allows data collection, storage and maintenance and collaboration with remote resea.
How it works?
What are the advantages of the TMA system?
save
Reagent
Time
Tissue
Workforce
Decreased assay volume
Allow
Standardized laboratory conditions through automation
Central data handling
Simultaneous analyses of very large numbers of specimens
TMA technique conserves valuable tissue for further studies ad diagnosis
Allows formation of digital database of slides and images
High quality brightfield and fluorescent images
External links for additional information
Pannoramic MIDI II
TMA Grand Master
QuantCenter
for more information
